Modifying REGISTRY settings incorrectly can cause serious problems that may prevent your computer from booting properly. Microsoft cannot guarantee that any problems resulting from the configuring of REGISTRY settings can be solved. Modifications of these settings
are at your own risk.
Always backup the registry before making changes. See this article on how to backup and restore the registry:
How to back up and restore the registry in Windows http://support.microsoft.com/kb/322756
It is possible to manually edit the system registry to remove the AutoPlay handlers. The AutoPlay handlers are stored at the following registry location:
Above registry key stores various event names, which contains associated handlers. Which mean all entries added as the value for the event will be shown as an option when the particular event happens and trigger the AutoPlay menu dialog.