Problem Ejecting USB Mass Storage Device "This device is currently in use. Close any programs or windows that might be using the device, and then try again."

Hi A. Cook,

1)       How long have you been facing this issue?

2)       Is this issue confined to any specific USB device?

This behavior can occur if there are open handles with any files in the USB (Universal Serial Bus) Mass Storage Device are in use by another program.

 
Method 1:

a)       Download Process Explorer from the link below

http://technet.microsoft.com/en-us/sysinternals/bb896653

b)       Click Find, Find Handle or DLL (Dynamic Link Library) in the tool menu.

c)       Type the drive letter of the USB device in the Handle or DLL substring textbox, and press Search Button.

d)       Find the process and its PID (Process Identifier) in the following box.

e)       In system process tree view, find the process according to the Find Handle or DLL dialog box.

f)        Press Ctrl + H to show Handles in Lower Pane View.

g)       Find the File according with the drive letter, and right click it, choose Close Handle.

Reference:

Problem Ejecting USB Mass Storage Device
http://support.microsoft.com/kb/555665

Note: Using Third Party Software, including hardware drivers can cause serious problems that may prevent your computer from booting properly. Microsoft cannot guarantee that any problems resulting from the use of Third Party Software can be solved. Using Third Party Software is at your own risk.

Method 2:  You may refer the steps provided by Diana D in the link below
http://answers.microsoft.com/en-us/windows/forum/windows_vista-hardware/problem-ejecting-usb-mass-storage-deviceits-in-use/fd56805c-ed8e-491f-8a2b-39ed1c988a71

Method 3: Run an online scan for any threat and try to fix them

http://onecare.live.com/site/en-Us/center/cleanup.htm